217)
A urine sample of a 34-year-old female patient is examined by gas chromatography–mass spectrometry (GC-MS) after deglucuronidation, liquid/liquid extraction, and derivatization.
| Substance |
RT (min) |
Characteristic ions (m/z) |
LLOD (ng/mL) |
LLOQ (ng/mL) |
| Cocaine-D3 |
3.596 |
185, 201, 306 |
— |
— |
| EDDP-TMS |
3.201 |
277, 262, 220, 200 |
100 |
200 |
| Methadone |
3.445 |
72, 165, 223, 294 |
10 |
25 |
| Pentazocine-TMS |
3.734 |
274, 289, 342, 357 |
10 |
25 |
| Cocaine |
3.613 |
182, 198, 272, 303 |
10 |
25 |
| Cocaethylene |
3.758 |
196, 82, 317 |
10 |
25 |
| BEG-D3-TMS |
3.775 |
243, 259, 364 |
— |
— |
| BEG-TMS |
3.784 |
240, 361, 256 |
25 |
50 |
| Morphine-D3-2TMS |
4.351 |
432, 404, 417 |
— |
— |
| Codeine-TMS |
4.236 |
178, 196, 234, 371 |
10 |
25 |
| Morphine-2TMS |
4.360 |
429, 236, 414, 401 |
10 |
25 |
| O-6-MAM-TMS |
4.499 |
399, 287, 340, 355 |
10 |
25 |
| Norbuprenorphine-2TMS |
5.627 |
468, 500, 510, 524, 557 |
2 |
5 |
| Buprenorphine-TMS |
6.065 |
450, 482, 506, 524, 539 |
2 |
5 |
| THC-COOH-D3-2TMS |
4.770 |
374, 476, 491 |
— |
— |
| THC-COOH-2TMS |
4.777 |
371, 473, 488 |
5 |
10 |
| Fentanyl-D5 |
4.798 |
250, 194 |
— |
— |
| Norfentanyl-TMS |
3.230 |
155, 154, 247, 289, 304 |
10 |
25 |
| Fentanyl |
4.800 |
245, 146, 189, 202 |
5 |
10 |
| Sufentanyl |
4.927 |
289, 140, 187, 238, 289 |
2 |
5 |
| Alfentanyl |
5.185 |
289, 222, 268, 359 |
5 |
10 |
From Strano-Rossi S, Bermejo AM, de la Torre X, Botrè F. Fast GC-MS method for the simultaneous screening of THC-COOH, cocaine, opiates and analogues including buprenorphine and fentanyl, and their metabolites in urine. Anal Bioanal Chem 2011;399:1623–1630.
Which one of the following compounds was definitely present in this patient’s urine?
